Histopathological and immunohistochemical evaluation of cellular response to a woven and electrospun polydioxanone (PDO) and polycaprolactone (PCL) patch for tendon repair.
Rashid, Mustafa, Dudhia, Jayesh, Dakin, Stephanie G, Snelling, Sarah J B, Ferro de Godoy, Roberta, Mouthuy, Pierre-Alexis, Smith, Roger K W, Morrey, Mark and Carr, Andrew J (2020) Histopathological and immunohistochemical evaluation of cellular response to a woven and electrospun polydioxanone (PDO) and polycaprolactone (PCL) patch for tendon repair. Scientific Reports, 10 (1). p. 4754. ISSN 2045-2322
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Abstract
We investigated endogenous tissue response to a woven and electrospun polydioxanone (PDO) and polycaprolactone (PCL) patch intended for tendon repair. A sheep tendon injury model characterised by a natural history of consistent failure of healing was chosen to assess the biological potential of woven and aligned electrospun fibres to induce a reparative response. Patches were implanted into 8 female adult English Mule sheep. Significant infiltration of tendon fibroblasts was observed within the electrospun component of the patch but not within the woven component. The cellular infiltrate into the electrospun fibres was accompanied by an extensive network of new blood vessel formation. Tendon fibroblasts were the most abundant scaffold-populating cell type. CD45, CD4 and CD14 cells were also present, with few foreign body giant cells. There were no local or systemic signs of excessive inflammation with normal hematology and serology for inflammatory markers three months after scaffold implantation. In conclusion, we demonstrate that an endogenous healing response can be safely induced in tendon by means of biophysical cues using a woven and electrospun patch.
| Item Type: | Article |
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| Divisions: | Equine & Veterinary Physiotherapy |
| Depositing User: | Dr Roberta Blake |
| Date Deposited: | 24 Nov 2020 16:54 |
| Last Modified: | 10 Nov 2023 11:49 |
| URI: | https://writtle.repository.guildhe.ac.uk/id/eprint/15189 |
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